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Proteolytic activities determined in colon samples. Trypsin‐like activity was measured using Boc‐Gln‐Ala‐Arg‐AMC (in A) and n‐Tos‐Gly‐Pro‐Arg‐AMC (in B). To determine the neutrophil elastase (in C), pancreas elastase (in D), KLK (in E) and chymotrypsin‐like activity (in F), Suc‐Ala‐Ala‐Pro‐Val‐AMC, Suc‐Ala‐Ala‐Ala‐AMC, H‐Pro‐Phe‐Arg‐AMC and Suc‐Ala‐Ala‐Pro‐Phe‐AMC were used respectively. Data are presented as mean ± SEM (n = 12). *P < 0.05; Mann–Whitney U‐test.
