Figure 6.

CAT639 inhibited dimerization of iNOS. (A) CAT639 slightly inhibited iNOS mRNA expression (n = 3). (B) CAT639 inhibited NO production induced by IL‐1 alone or IL‐1β + IFN‐γ. NO was measured using a nitrate/nitrite colorimetric assay kit (n = 3). (C) CAT639 had no effect on apoptosis induced by SNAP (n = 3). (D) CAT639 destabilized iNOS dimer in rat insulin‐producing INS1E cells. Dimeric proteins were determined by performing low‐temperature SDS‐PAGE. Samples lysed in sample buffer with β‐ME and boiled for 5 min were used as a positive control of total iNOS. Data are presented as mean ± SD.