Table 1.

Kinetic parameters of XacAbf51 and TxAbfD3 on pNP-Araf and arabinan and comparative activity of XacAbf51 and TxAbfD3 on arabinoxylan.

	pNP-Araf			Arabinan			Arabinoxylana
	Km (mM)	kcat (s−1)	kcat/Km (s−1 M−1)	Km (mg mL−1)	kcat (s−1)	kcat/Km (s−1 mg−1 mL)	v0/[E]t (s−1)
XacAbf51	0.19 ± 0.01	56.4 ± 0.8	3 × 105	20.4 ± 7.8	56.7 ± 6.4	2.8	9.1 ± 0.55
TxAbfD3	0.52 ± 0.06	154.2 ± 4.2	3 × 105	41 ± 9.5	27.15 ± 2.2	0.7	30.0 ± 1.31

For comparative purposes, the assays were performed at 50 °C, pH 5.5, which is compatible with industrial processes of biomass saccharification. The K_m and k_cat could not be estimated for arabinoxylan because the maximum velocity is not reached at the highest possible concentration of the substrate

^aActivity measured using substrate at 10 mg mL⁻¹