NPR2 antagonist administration increases brain infarct size and neuronal death after neonatal HI. NPR2 antagonist P19 was administered into the ipsilateral hemisphere via i.c.v. injection prior to HI, then HI insult was conducted. (A) Representative images of TTC staining and quantification of brain infarct size 48 h after HI. Data are expressed as mean ± SEM. n = 8 pups/group. *, p < 0.05 vs. vehicle. Two-tailed Student’s t-test. (B) Representative confocal images of the colocalization of TUNEL (red) with neurons (NeuN, green) in the cortex of the ipsilateral hemisphere of the brain 48 h after HI. Scale bar, 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)