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. 2018 Aug 7;26(8):1101–1115.e6. doi: 10.1016/j.str.2018.05.014

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Crown Copyright © Published by Elsevier Ltd. All rights reserved.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Activation of NDR1 Kinase by MOB1 and/or a Phosphorylated HM Peptide

(A) In vitro kinase assay analysis of wild-type GST-NDR1^MBD−KD in the presence and absence of MOB1 activator and/or a phospho-HM peptide in trans. The top two panels show the autoradiograph, and the bottom three panels show protein loading by Coomassie staining.

(B) In vitro auto-phosphorylation analysis of NDR1^MBD−KD versus NDR1^KD in the presence and absence of MOB1 and/or a phospho-HM peptide in trans. The top panel displays the autoradiograph, and the bottom two panels show protein loading by Coomassie staining.

(C) In vitro auto-phosphorylation and phosphorylation of RAB3IP substrate by NDR1^MBD−KD and NDR1^KD, in the presence and absence of MOB1 and/or a phospho-HM peptide in trans. The top two panels display autoradiographs for NDR1 auto-phosphorylation and RAB3IP phosphorylation, respectively. The bottom three panels show protein loading by Coomassie staining.