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Michaelis-Menten Kinetic Analysis of the Phosphorylation of the NDR1 Substrate RAB3IP by NDR1MBD−KD
Phosphorylation kinetic analysis of NDR1MBD−KD wild-type (top panel), Y331A mutant (middle panel), and 5A mutant (bottom panel) in the absence of MOB1 (left column) or in the presence of MOB1 (right column). n = 3 experiments done in technical duplicate; error bars, SEM.
