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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2018 May 22;29(9):1802–1811. doi: 10.1007/s13361-018-1969-z

Figure 1.

Figure 1

4-bit binary representation for both CS and HS/Hp glycan disaccharides. Each bit is turned on (assigned 1) if a modification is present and off (assigned 0) if the R-group is a hydrogen. Bit 2 represents R2 which has an acetyl modification instead of a hydrogen for an off-bit assignment. In the case of HS where the free-amine is possible, a different numeral can be used to represent the absence of SO3 and acetylation. Additional bits can be introduced so serve as negative control bits as well as a representation for the uronic sugar stereochemistry.