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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Epilepsy Res. 2018 Jul 21;145:169–177. doi: 10.1016/j.eplepsyres.2018.07.003

Fig. 4. Laforin N163D loses the ability to interact with recognized partners.

Fig. 4

Yeast strain THY-AP4 was transformed with the following combination of plasmids: in the left panel, plasmids expressing LexA-laforin wild type were combined with plasmids pGAD-empty or pGAD-laforin WT, in order to check dimerization; similarly plasmid expressing LexA-laforin N163D was combined with pGAD-empty or pGAD-laforin N163D. In the right panel, plasmids expressing LexA-laforin wild type or N163D were combined with and plasmids pGAD-empty, pGAD-R5, pGAD-R6 and pGAD-malin respectively. The strength of the interaction was determined by measuring β-galactosidase activity as indicated in Material and Methods. Results are the mean of at least six independent transformants in each case (bars indicate standard deviation; ***p<0.001). A representative Western blot of the proteins expressed by the different transformants (two samples of each combination) is shown in the lower panel. Western blot was analyzed with anti-LexA antibodies.