Skip to main content
. 2018 Aug;18(3):252–259. doi: 10.17305/bjbms.2018.2376

FIGURE 3.

FIGURE 3

Ectopic expression of microRNA 466 (miRNA-466) inhibits proliferation, induces cell cycle arrest and apoptosis in colorectal cancer (CRC) cells. SW-620 cells were transfected with miR-466 mimics or negative control miRNA (Neg-miR). (A) Increased levels of miR-466 were confirmed by quantitative reverse transcription polymerase chain reaction in the cells transfected with miR-466 mimics compared to Neg-miR-transfected cells (p < 0.001). (B) CCK-8 assay showed significantly decreased proliferation rates in miR-466 mimics-transfected cells at 48 hours and 72 hours (p < 0.05 and p < 0.01, respectively) compared to the control. (C) PI staining showed that the percentage of cells in G0/G1 phase was significantly increased, from 54.45% in Neg-miR-transfected cells to 68.01% in miR-466 mimics-transfected cells (p < 0.001). Moreover, the overexpression of miR-466 remarkably decreased the percentage of SW-620 cells in S and G2/M phase (p < 0.001 and p < 0.05, respectively). (D) Flow cytometry analysis showed that the early apoptosis and late apoptosis rates were significantly increased in SW-620 cells transfected with miR-466 mimics compared to Neg-miR-transfected cells (p < 0.001 and p < 0.05, respectively). Values are expressed as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Neg-miR group.