Table 1.

Custom qPCR primers for determination of transcript abundance

Gene ID (Ref Seq)	Primers (Forward and Reverse)	Product Length, bp	Melting Temp, °C
Tmem16a NM_178642.5	F: TCTGTGTTTATGGCCCTCTGG R: TGACAGCTTCCTCCTCCTCC	115	F: 59.72 R: 60.62
Best2 NM_001130194.1	F: CCGCCTATCGCTTCTTACTGG R: GATGCACCACCAGAGTCACG	132	F: 60.60 R: 61.02
Cftr NM_021050.2	F: ATTTCACGCTCCACAGAGGC R: CCATTAACGGGGTTGTTTTTAAGC	118	F: 60.67 R: 59.31
Chrm3 NM_033269.4	F: CTGCCAGATATGACCAGCAATGG R: TCACTTGGTCAGAACGCAGC	141	F: 61.61 R: 60.88
Itpr3 NM_080553.3	F: ACATTGTGTCCCTGTACGCC R: CACTTTGAAGAGGCAATCTCGG	143	F: 60.32 R: 59.58

All primers were designed utilizing the NCBI primer-blast application with the indicated reference sequence (Ref Seq). Best2, bestrophin-2; Chrm3, cholinergic receptor muscarinic 3; F, forward; Itpr3, inositol trisphosphate receptor; R, reverse; Temp, temperature.