Fig. 3.

Outflow facility data from human anterior segments exposed to 1 nM endothelin-1 (ET-1). A: perfusion trace summary showing combined outflow facility data from 3 pairs of human anterior segments, normalized to baseline measurements. ET-1 (100 nM) was exchanged into both anterior segments of the pair at ~2 h. Diethylenetriamine-nitric oxide (DETA-NO; 100 µM) was then exchanged into the experimental segment at ~4 h in the presence of ET-1 (1 nM), whereas ET-1 (1 nM) was exchanged into the contralateral segment. Data are presented as mean ± SD (solid lines ± shaded regions). B: box and whisker plot summarizing the facility values obtained at each stage of the human anterior segment perfusions. Data show an average (represented by the + in the box of each data set) facility value of 0.23 ± 0.06 µl·min⁻¹·mmHg in anterior segments with the trabecular meshwork (TM) intact reported in previous studies [^Ψthe outflow facility data were obtained from 73 anterior segments that had been perfused and previously published by our laboratory (8, 13, 46, 55, 56)]. Trabeculotomized eyes (TRAB) showed a facility value of 0.61 ± 0.12 µl·min⁻¹·mmHg (control and experimental data). ET-1 treatment decreased facility to 0.40 ± 0.15 µl·min⁻¹·mmHg (control and experimental data), and DETA-NO reversed this decrease in facility, achieving a value of 0.54 ± 0.15 µl·min⁻¹·mmHg (experimental data). The box represents the range of the values from the 25th to the 75th percentile, whereas the error bars show the minimum and maximum values of each data set. The horizontal line in the box represents the median value.