Fig. 4.

Combined and normalized facility data from porcine anterior segment perfusions. A: combined perfusion data showing outflow facility in 8 porcine anterior segment pairs normalized to baseline. Endothelin-1 (ET-1; 100 nM) was exchanged into both anterior segment chambers of the pair at ~2 h. Diethylenetriamine-nitric oxide (DETA-NO; 100 µM) was then exchanged into the experimental chamber at ~4 h in the presence of ET-1 (1 nM), whereas ET-1 (100 nM) was exchanged into the contralateral segment. Data are presented as mean ± SD (solid lines ± shaded regions). B: box and whisker plot showing the facility values collected at each stage of the porcine anterior segment perfusions. Data show a facility value of 0.28 ± 0.13 µl·min⁻¹·mmHg in anterior segments with the trabecular meshwork (TM) intact (control and experimental data). Trabeculotomized eyes (TRAB) showed a facility value of 0.72 ± 0.32 µl·min⁻¹·mmHg (control and experimental data). ET-1 treatment decreased facility to 0.48 ± 0.21 µl·min⁻¹·mmHg (control and experimental data), and DETA-NO reversed this decrease in facility achieving a value of 0.73 ± 0.21 µl·min⁻¹·mmHg (experimental data). The mean is indicated by the cross in the box. The box represents the range of the values from the 25th to the 75th percentile, whereas the error bars show the minimum and maximum values of each data set. The horizontal line in the box represents the median value.