Table 3.

Animal characteristics of muscle gp130 KO mice following 2 wk of systemic IL-6 overexpression

	WT	KO
	Vector	Vector	IL-6
No. of mice	7	6	7
Body weight, g
Pre	24.8 ± 0.5	26.3 ± 0.8	25.6 ± 0.6
Post	25.7 ± 0.4	27.0 ± 0.8*	26.4 ± 0.5*
%Change from Pre	3.9 ± 1.9	2.8 ± 0.6	2.8 ± 0.6
Tibialis anterior, mg	47 ± 0.6	49 ± 1.4	49 ± 1.0
Epididymal fat, mg	274 ± 9	295 ± 26	284 ± 23
Spleen, mg	87 ± 5	76 ± 4	137 ± 3†
Testes, mg	185 ± 9	211 ± 4‡	222 ± 6
LABC, mg	90 ± 2	85 ± 2	84 ± 1
Seminal vesicle, mg	259 ± 12	257 ± 15	273 ± 16
Plasma IL-6, pg/ml	0 ± 0	0 ± 0	200 ± 34†
Tibia length, mm	16.8 ± 0.1	17.0 ± 0.1	16.9 ± 0.1

Data are means ± SE. Wild-type (WT) and knockout (KO) littermates subjected to control Vector electroporation were compared by Student’s t-test to determine differences with gp130 loss. A two-way ANOVA (IL-6 × time) was used to determine differences in body weight over time between Vector and IL-6 within KO mice. Student’s t-test was performed to determine differences in all other variables between Vector and IL-6 within KO mice. Statistical significance was set at P < 0.05. LABC, levator ani/bulbocavernosus muscle.

^*Main effect of post time point;

^†different from KO Vector;

^‡different from WT Vector.