Fig. 5.

Knockdown of mitofilin in rat H9c2 myoblasts increases poly(ADP-ribose) polymerase (PARP) cleavage and induces cell cycle arrest. A: immunoblot and graph showing an increase in cleaved PARP in H9c2 cells transfected with mitofilin siRNA-1 versus scrambled siRNA. B: H9c2 cells transfected with mitofilin siRNA-1 or scrambled siRNA were stained with propidium iodide (PI) for cell cycle analysis. The result indicates that mitofilin-deficient H9c2 cells have much longer S and G₂ phases compared with scrambled siRNA indicating arrest in the cell cycle. Values are expressed as means ± SE. *P < 0.05 H9c2 cells treated with mitofilin siRNA-1 versus cells treated with scrambled siRNA (n = 3/group).