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. 2018 Feb 28;315(1):C28–C43. doi: 10.1152/ajpcell.00230.2017

Fig. 8.

Fig. 8.

Transfection of human embryonic kidney (HEK) 293 cells with mitofilin siRNA reduces mitofilin expression. A: Western blot analysis showing the reduction in expression of mitofilin in HEK 293 cells after transfection with mitofilin siRNA-1 versus scrambled siRNA. Values are expressed as means ± SE. *P < 0.001 mitofilin siRNA-treated cells versus cells treated with scrambled siRNA (n = 10/group). B: immunoblot and graph showing an increase in apoptosis-inducing factor (AIF) expression in HEK 293 cells transfected with mitofilin siRNA-1 versus scrambled siRNA. C: immunoblot and graph showing no change in cleaved caspase 3 in HEK 293 cells transfected with mitofilin siRNA-1 versus scrambled siRNA. PARP, poly(ADP-ribose) polymerase. D: immunoblot and graph showing an increase in cleaved PARP in HEK 293 cells transfected with mitofilin siRNA-1 versus scrambled siRNA. Values are expressed as means ± SE. *P < 0.05 HEK 293 cells treated with mitofilin siRNA-1 versus cells treated with scrambled siRNA (n = 3/group).