Table 1.

Sequences of all end‐point PCR primers sets, plus number of cycles, denaturing temperature, and the expected product sizes

Primer	Sequence	Number of cycles	Denaturing temperature (°C)	Product size (bp)
UT‐A F5	5′ – GTGGCTTCTGTTTCCTGTGACCTT – 3′	35	60	UT‐A1/3
UT‐A R10	5′ – CCTCGGGGTAGGTGACTTTGCTGAGT – 3′	35	60	F5/R10 = 682
UT‐A F23	5′ – CTGTTCATATCCTCACCTCTCATTTG – 3′	35	60	UT‐A1/2
UT‐A R25	5′ – CACAGATAACATGTTAGCCAGGGC – 3′	35	60	F23/R25 = 252
UT‐B F1	5′ – GCCAGGAAGCCAGCTAGAGTGGTC – 3′	35	60	UT‐B1 or UT‐B2
UT‐B R5	5′– CTGTCCTGGCTGAGCAAGAGG – 3′	35	60	F1/R5 = 450 or 600
UT‐B F4	5′ – CATCTTGCCAACCAGCTTAAAG – 3′	35	60	UT‐B
UT‐B F6	5′ – GGTGGGAGTACTCATGGCTGTCTTTTC – 3′	35	60	F4/R5 = 219
UT‐B R6	5′ – GTAACAGCCACCAGAAATAGTC – 3′	35	60	F4/R6 = 320
UT‐B R10	5′ ‐ GCCATAAAGTTTGCCATGCCG ‐ 3′	35	60	F4/R10  = 870 F6/R10 = 609
Actin_F	5′ – GTGCTGTCTGGCACCACCAT – 3′	30	55	Actin
Actin_R	5′ – CCTGTAACAACGCATCTCATAT – 3′	30	55	514

KEY: F = forward primer; R = reverse primer; “F5” = forward primer targeted to exon 5 etc.