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. 2018 Aug 6;9:1742. doi: 10.3389/fmicb.2018.01742

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Copyright © 2018 Tenconi, Traxler, Hoebreck, van Wezel and Rigali.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Prodiginine biosynthesis causes massive loss of viable filaments. SYTOX (blue) and SYTO9 (green) staining of cross sections of culture of S. coelicolor M145 (wild-type, WT) and its redD null mutant inoculated in R2YE agar plates. Bars, 40 μm. Note that (i) the PCD round prior to PdG production also occurs at 30 h in the redD null mutant of S. coelicolor, and (ii) the higher accumulation of viable filaments (only stained by SYTO9) in the redD mutant. Quantification of the SYTOX and SYTO9 fluorescence signals are displayed in plots next to the microscopy pictures.