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. 2018 Aug 6;9:865. doi: 10.3389/fphar.2018.00865

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Copyright © 2018 Diezmos, Markus, Perera, Gan, Zhang, Sandow, Bertrand and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transepithelial electrical resistance (TEER) in Caco-2 cells. Data were expressed as the percentage change of TEER relative to pre-treatment of the same well. Two-way ANOVA analysis followed by Holm–Sidak’s multiple comparison tests showed a highly significant reduction in TEER values after Caco-2 cells were incubated with cytokines TNFα and IL-1β (each 100 ng/mL) for 20 h and 48 h (^∗∗∗∗P < 0.0001 compared to control). (A) Cytokines-induced TEER reduction was significantly prevented by co-incubation with ¹⁰Panx1 (100 μM). (B) The rise of cytokines-induced epithelial cell permeability was inhibited by the combined treatment with A438079 (100 μM) and the pannexin/connexin blocker CBX (100 μM), but A438079 and CBX on their own showed no protective effect. Each condition was measured as duplicates and repeated three to eight times.