FIGURE 2.

Palmitate-enriched diet increases α-syn expression levels in m-Thy1-mice. Representative western blot (A) and optical density (B) of α-syn in the substantia nigra-enriched fraction of brains from m-Thy1-αsyn mice showing that the PA diet significantly increases α-syn. (C) Real-time RT-PCR shows that the PA diet also increases SNCA mRNA. Immunocytochemistry of the substantia nigra shows that PA diet-fed mice (E) exhibit more immunoreactivity to α-syn antibody than control diet fed mice (D). ^∗p < 0.05 versus control diet.