FIGURE 3.

B6D2 mice on a PA diet exhibit increased TH and pS⁴⁰TH expression. Representative western blot (A,C) and optical density (B,D) showing the PA diet significantly increases levels of TH and pS⁴⁰TH respectively in the substantia nigra-enriched fraction of brains from B6D2 mice. Real-time RT-PCR shows that the PA diet significantly increases TH mRNA (E). Immunocytochemistry shows that PA diet-fed mice exhibit increased immunoreactivity to TH antibody (F) compared to control diet-fed (G). ^∗p < 0.05 versus control diet.