Figure 7.

The interaction between CD36 and microcystin. (A) The exposure of microcystin, decrease expression of (i) SREBF1 mRNA, (ii) decrease expression of HNF4A mRNA; decreases degradation of (iii) of NFE2L2; Increases the expression of (iv) RPS27A; increases the cleavage of (v) PARP1 protein. (B) Microcystin differentially regulates PARP1 and HNF4α expression in A427 lung cancer cell line and in Cyanobacteria positive LUAD tissue samples. A427 cells were treated with various concentrations of Microcystin for 48 h, total cell lysate extracted and analyzed by immunoblotting using antibodies specific for PARP1 and HNF4α, (also see Figure S4). (C) Protein lysate was extracted from fresh frozen tissue-matched samples of patient positive for Cyanobacteria and immunoblotting analysis performed using a PARP1 antibody, (also see Figure S3).