FIGURE 2.

Co-expression of Ldb1 and cell-type markers in P21 wildtype retinas. (A) Pax6 and Ldb1 are co-labeled in horizontal and amacrine cells as well as in cells within the GCL. (B) Many but not all Ldb1⁺ cells are co-labeled with Lmo4, and vice versa. (C–E) Ldb1 immunoreactivity is seen in the great majority of syntaxin⁺ amacrine cells, GAD67⁺ GABAergic amacrine subtypes, and GLYT1⁺ glycinergic amacrine subtypes. (F,G) Ldb1 immunoreactivity is seen in the great majority of Chx10⁺ bipolar cells and PKCα⁺ rod bipolar subtypes. (H) Ldb1 immunoreactivity is seen in the Bhlhb5⁺ type 2 OFF-cone bipolar cells and GABAergic amacrine cells. (I,J) All calbindin⁺ or Lhx1⁺ horizontal cells are co-labeled with Ldb1. (K) Ldb1 is expressed in all Brn3a⁺ ganglion cells. (L) Ldb1 and glutamine synthetase (GS) are co-expressed in most Müller cells. (M) Percentages of marker-positive retinal cells that are immunoreactive for Ldb1. Each histogram represents the mean ± SD for three retinas. All retinal sections were counterstained with nuclear DAPI. Arrows point to representative co-labeled cells. Inset pictures in the lower right corners are magnified from corresponding outlined regions. GCL, ganglion cell layer; HC, horizontal cell; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer. Scale bar: (A–L) 28.6 μm.