Fig. 4.

Competition experiments. PKD kidneys from jck (A) and Thm1-conditional knockout (cKO) (B) mice were loaded on CR8-agarose beads in the presence of vehicle (DMSO), 250 µM roscovitine or CR8 (both interact with CK1 and CDK5), or 50 µM D4476, IC261 (general CK1 inhibitors), or PF 4800567 (CK1ε specific inhibitor) (the latter 3 do not interact with CDK5). After extensive washing, the bound proteins were analyzed by SDS-PAGE and WB, with antibodies directed against CK1ε, CK1α and CDK5. Note that all products reduce the binding of CK1ε and CK1α, whereas only roscovitine and CR8 compete for CDK5 binding.