Fig. 5.

CK1ε is overexpressed in multiple PKD models. Extracts were prepared from kidneys of mouse PKD and corresponding WT animals and analyzed directly (crude extract) or following affinity purification on CR8-agarose beads. SDS-PAGE was followed by WB with antibodies directed against CK1ε. The nonpolycystic nephronophthisis (Nphp 4) model, used as a control, displayed no change in CK1ε expression. Thin lines in the blots indicate the position of the 51-kDa molecular weight marker (MWM). Crude extracts and CR8-purified proteins, separated by a MWM lane, were run on the same gel, and a single WB blot was carried out for all 4 samples for each mouse model. In Cpk samples, because of important signal intensity differences, different exposures were necessary for crude extracts and CR8-purified proteins. The 2 WB, assembled in a single panel, are thus separated by a vertical dotted line. In Pkd2-cKO samples, crude extracts and CR8-agarose were not separated by MWM, and the WB was cut and separated by a blank space delineated by a vertical dotted line.