Fig. 9.
Alteration in CK1ε and CK1α expression in kidneys of PKD models. A and B: CK1ε and CK1α mRNA levels were quantified in kidneys, liver, heart, and brain of jck (A) and Pkd1-cKO (B) mice. PCR data were normalized to TBP mRNA levels and are presented as fold change of polycystic over WT tissue levels. C and D: CK1ε and CK1α protein levels were estimated by WB in kidneys, liver, heart, and brain of jck (C) and Pkd1 cKO (D) mice. Equal amounts of crude extracts of WT and PKD mouse tissues were loaded and resolved on SDS-PAGE, and proteins were detected by WB with specific antibodies. GAPDH was used as a loading control. Increased expression of CK1ε and altered CK1α pattern were seen only in the polycystic kidneys compared with WT kidneys. The decrease in p21cip1 was confirmed in the kidneys of both models; n = 1 for each organ. Tested in triplicate. *P = 0.01–0.05, **P = 0.001–0.01, and ***P < 0.001 (Student’s t-test).