Fig. 8.
PKC inhibition does not reduce Cl− absorption (JCl) in cortical collecting ducts (CCDs) from STE20/SPS-1-related proline-alanine-rich protein kinase (SPAK) null mice. CCDs from SPAK null mice (n = 4) were perfused in vitro. Each CCD was perfused in the presence and absence of the PKC inhibitor GF109203X at a concentration of either 2 or 4 μM. Solid lines show data from tubules where the vehicle was present in the 1st period and GF109203X in the 2nd period. Dashed lines show data from tubules where GF109203 was present in the 1st period and then removed (i.e., washed out) in the 2nd. As shown, GF109203X did not change JCl in SPAK null CCDs. Squares indicate means ± SE under each condition.