Fig. 3.

Tubular injury during and after repeated cisplatin (CDDP) treatment. Eight-week-old FVB mice were treated with either saline vehicle or CDDP (7 mg/kg) once per week for 2 wk and euthanized 3 days after dose 2. Another group was treated with either saline vehicle or CDDP (7 mg/kg) once per week for 4 wk and euthanized 3 days after dose 4, or allowed to age for 6 mo following treatment (6 mo post-CDDP). Severity of tubular dilatation (A), loss of brush border (B), tubular degeneration (C), tubular casts (D), tubular necrosis (E), inflammation (F), and distal damage (G) was determined by a renal pathologist. Data are expressed as means ± SE; n = 5–7. Statistical significance was determined by 2-way ANOVA followed by Tukey posttest. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.