Fig. 6.

Profibrotic growth factor production during and after repeated cisplatin (CDDP) treatment. Eight-week-old FVB mice were treated with either saline vehicle or CDDP (7 mg/kg) once per week for 2 wk and euthanized 3 days after dose 2. Another group was treated with either saline vehicle or CDDP (7 mg/kg) once per week for 4 wk and euthanized 3 days after dose 4, or allowed to age for 6 mo following treatment (6 mo post-CDDP). mRNA levels of transforming growth factor β (Tgfβ) (A) and connective tissue growth factor (Ctgf) (B) were measured in the kidney cortex via qRT-PCR. Data are expressed as means ± SE; n = 5–10. Statistical significance was determined by 2-way ANOVA followed by Tukey posttest. *P < 0.05, **P < 0.01.