Fig. 10.

Overview of pathological changes that occur during and after repeated cisplatin (CDDP) treatment. Mice treated with 2 doses of CDDP developed albuminuria, mild tubular pathologies, slight inflammation, and mild endothelial dysfunction. There was also increased level of NGAL in urine. Mice treated with 4 doses of CDDP and euthanized at day 24 still had albuminuria. In these mice, NGAL levels peaked and there were more severe tubular pathologies, as well as endothelial dysfunction. In addition, mice treated with 4 doses of CDDP and euthanized at day 24 developed interstitial fibrosis, which coincided with an increase in total macrophages and increase in profibrotic growth factors. After 6-mo age out, mice treated with 4 doses of CDDP no longer had detectable levels of albuminuria or NGAL, but inflammation and interstitial fibrosis worsened. In addition, these mice began to develop periglomerular fibrosis.