Fig. 6.

Proliferation in labeled cells of renin lineage (CoRL). BrdU was administered twice weekly to measure cumulative proliferation. Double staining for BrdU (green) and RFP (red) was performed to measure the percentage of proliferating labeled CoRL (yellow from merge of colors). A–H: representative pictures of staining for BrdU, RFP, and DAPI in young and aged mice following 25 days of treatment. I: graph shows data for individual mice at D25 in water (n = 4 in young, n = 6 in aged), hydralazine (n = 6 in young, n = 4 in aged), enalapril (n = 6 in young, n = 6 in aged), and losartan (n = 5 in young, n = 7 in aged) treatment group. Scale bars = 50 μm.