Fig. 3.

Let-7b inhibit Wnt/β-catenin signaling. (A) HepG2 HCC cells were co-transferred with Wnt/β-catenin luciferase-reporter construct and let-7b or con-miR. Wnt/β-catenin activity was normalized to Renilla luciferase activity. (B) β-catenin, c-Myc and E-cadherin mRNA level in HepG2 HCC cells after let-7b overexpression; (C) β-catenin, c-Myc and E-cadherin protein expression was determined by WB. β-Actin was used as an internal control. Results are mean ± SD (n = 3). ^*p < 0.05 vs BC; ^**p < 0.05 vs con-miR.