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PCR conditions for genotyping
| Step | Temperature, °C | Time | Remark |
|---|---|---|---|
| 1 | 94 | 2 min | |
| 2 | 94 | 20 s | |
| 3 | 65 | 15 s |
Decrease in temperature by 0.5°C per cycle |
| 4 | 68 | 10 s | |
| 5 |
Repeating steps 2 to 4 for 10 cycles |
||
| 6 | 94 | 15 s | |
| 7 | 60 | 15 s | |
| 8 | 72 | 10 s | |
| 9 |
Repeating steps 6 to 8 for 28 cycles |
||
| 10 | 72 | 2 min | |
| 11 | 10 | Stop |
