Fig. 2.
Expression of Cre-dependent channelrhodopsin in proopiomelanocortin (Pomc)-Cre cells does not overlap with Pomc-green fluorescent protein (Gfp) cells in the nucleus of the solitary tract (NTS). Representative confocal image showing that Pomc-Gfp cells do no colocalize with cells expressing Cre recombinase-dependent ChR2mCherry (magenta) in slices taken from mice expressing both Cre recombinase and Gfp driven by Pomc promoter sequences. An adeno-associated viral vector (AAV) encoding Cre recombinase-dependent ChR2mCherry was injected in the NTS 17 days before perfusion. Rostral (R) and lateral (L) orientations are indicated. Scale bar: 50 μm.