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. 2018 Mar 15;315(1):L102–L115. doi: 10.1152/ajplung.00511.2017

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Fig. 3. — Targeted neuropilin-1 (nrp1) deletion preferentially alters transgelin (tagln)-expressing mesenchymal cells lacking platelet-derived growth factor (PDGF)-α receptor [PDGFRα (CD140a)]. A−D: fibroblasts were isolated at postnatal day 8 from 5 TGCre^+/−;Nrp1F/F mice and 5 littermate TGCre^+/−;Nrp1F/⁻ controls (obtained from 4 separate litters) and analyzed as described in Fig. 1 legend, including negative selection for CD45; however, PDGFRα-containing cells were identified by staining with anti-CD140a (PDGFRα). Ordinate scale shows proportion of a population (designated along the abscissa) that stains for a particular marker: PDGFRβ (A), α-smooth muscle actin [α-SMA (B)], nestin [NES (C)], and desmin [DES (D)]. E: in a different cohort of 4 TGCre^+/−;Nrp1F/F mice and 4 littermate controls, isolated mesenchymal cells were stained with neural/glial antigen-2 (NG2) and PDGFRα (CD140a) and analyzed by fluorescence-activated cell sorting. Box-and-whisker (Tukey) plots show data from 5 (A−D) or 4 (E) separate isolations. CD140a^neg and CD140a^pos populations were compared across genotypes by 2-way ANOVA.