Fig. 3.

AKT3 is a direct target of miR-582-5p. (A) AKT3 expression level was detected by Taqman qRT-PCR. ^*P < 0.05 vs normal tissues; (B) AKT3 mRNA level was determined by Taqman qRT-PCR after miR-582-5p overexpression; (C) 3′UTR luciferase assay. The ECC1 cells were co-transfected with AKT3 3′ UTR or negative control construct (Ctrl) reporter with miR-NC or mir-582-5p. Luciferase assays were performed after 48hrs. ^*p < 0.05 vs miR-NC; (D) AKT3 protein level was analyzed by western blotting after overexpression of miR-582-5p for 48 h.