Fig. 4.

AKT3 upregulation obligated the effect of miR-582-5p on cells. (A) AKT3 mRNA level after ATK3 upregulation in the ECC1 cells overexpressing miR-582-5p was detected by Taqman qRT-PCR. ^*P < 0.05 vs VC; (B) AKT3 protein level was determined by western blotting after AKT3 upregulation in ECC1 cells overexpressing miR-582-5p; (C) cell proliferation was analyzed with Cyquant assay. ECC1 cells were transfected with ATK3 construct to overexpress AKT3 after miR-582-5p upregulated in ECC1 cells. Luciferase assays were performed after 48 h; (D) Cell growth after AKT3 overexpression was evaluated by 2D colony formation assay; (E) AKT3 effect on cell apoptosis was detected by Annexin V assay. ^*P < 0.05 vs miR-582-5p; P < 0.05 vs miR-582-5p + VC.