Figure 1.

Establishment of Organoid Cultures from Needle Biopsies of Hepatocellular Carcinoma and Paired Non-tumor Liver Tissues

(A) Schematic workflow of organoid generation from needle biopsies.

(B) Representative biopsy pieces of tumor and liver tissue used for organoid generation.

(C) Representative bright-field images of tumor and paired non-tumor liver tissue organoids from three different patients. Tumor organoids form compact spheroids, whereas liver organoids from non-tumor liver tissue grow as cystic structures. Organoids were imaged at the indicated passage numbers. Scale bar: 500 μm.