Figure 5.

Repertoire of Genetic Alterations Found in the HCC and CCC Organoids and Their Originating Tumors

(A) Venn diagrams illustrate the number of somatic non-synonymous mutations present in each HCC biopsy and their derivative HCC organoids. The dashed line denotes CCC-derived tumors and corresponding organoids.

(B) Repertoire of somatic non-synonymous mutations affecting cancer genes (Fujimoto et al., 2016, Kandoth et al., 2013, Lawrence et al., 2014, Cancer Genome Atlas Research Network, 2017). The effects of the mutations are color-coded according to the legend, with hotspots (Chang et al., 2016, Gao et al., 2017) colored in red. Multiple non-synonymous mutations in the same gene are indicated by an asterisk. Loss of heterozygosity of the wild-type allele of a mutated gene is represented by a diagonal bar, and mutations found to be clonal by ABSOLUTE (Carter et al., 2012) are indicated by a black box.

(C) Contour plots illustrate the distribution of the cancer cell fractions (CCFs) of somatic mutations in the tumors and their corresponding organoids, with the increasing shades of red indicating higher number of somatic mutations at a given CCF.