FIG 6.
The carB mutant strain is actively transcribing and translating during growth arrest in E. coli. (A) Growth profiles of E. coli MG1655 and MG1655ΔcarB, each containing a plasmid-borne PlacZ::gfp fusion [called MG1655(pgfp) and MG1655ΔcarB(pgfp), respectively]. Strains were grown in LB medium at 37°C with aeration. (B and C) GFP fluorescence (excitation wavelength, 485 nm; emission wavelength, 528 nm) was determined throughout growth. IPTG (1 mM) was added at time zero (B) or after 360 min (C). Data represent means and standard deviations of triplicate measures.