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Detection and analysis of CITase. (A) CITase production by wild-type and single, double, and triple mutant strains. One unit of CITase was defined as the amount of enzyme that produced 1 nmol of the sum of CI-7, CI-8, and CI-9 per min from dextran at pH 5.5 at 40°C. Experiments were performed in triplicate, and the results are expressed as means ± standard deviations. (B) SDS-PAGE (left) and zymography (right) of CITase. The arrows indicate native CITase and the 96 kDa protein.
