TABLE 3.

Intracellular concentrations of key metabolites during exponential (12 h) growth of the wild-type (T-3040) and triple mutant (YT478) strains^a

Metabolite	Concn (pmol/OD unit)b
	Wild-type (T-3040)	Mutant (YT478)
SAMc	134 ± 11	274 ± 19
ATP	970 ± 45	1,431 ± 330
GTP	159 ± 45	304 ± 158
UTP	247 ± 9.7	457 ± 115
CTP	234	756 ± 71
Glucose 6-phosphate	207 ± 167	527 ± 229
Fructose 1,6-diphosphate	185 ± 147	1,000 ± 572
3-Phosphoglyceric acid	465 ± 226	1,565 ± 406
2-Phosphoglyceric acid	68 ± 42	157 ± 24
Phosphoenolpyruvic acid	133 ± 59	446 ± 77

^aStrains were grown to mid-exponential (12 h) phase in LB-Dex medium. Intracellular metabolites were extracted and subjected to metabolome analysis.

^bAll experiments were performed in triplicate, and the data are expressed as means ± standard deviations.

^cSAM, S-adenosylmethionine.