FIG 2.

Oligomeric state of PhTET4 in solution. (A) Gel filtration analysis of PhTET2 and PhTET4 dodecameric complexes on a Superose 6 column. Dashed line, PhTET2 dodecamer; solid line, PhTET4 dodecamer. The recombinant protein PhTET4 eluted in the same volume as the 450-kDa PhTET2 dodecameric complex. (B) PhTET2 (top) and PhTET4 (bottom) observed by negative-stain electron microscopy. Homogeneous populations of hollow triangular particles are observed at a magnification of ×49,000 for both PhTET2 and PhTET4 samples.