TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| E. coli TOP10 | Cloning strain | Invitrogen |
| H. pylori | ||
| 43504 | Parental strain | ATCCb |
| ΔureB mutant | ΔureB::KSF Kanr Sucs | This study |
| ΔureAB mutant | ΔureAB::cat Cmr | 15 |
| ureBH136A strain | Codon for His136 replaced by Ala | This study |
| ureBH138A strain | Codon for His138 replaced by Ala | This study |
| ureBK219A strain | Codon for Lys219 replaced by Ala | This study |
| Plasmids | ||
| pBluescript KS(+) | Cloning vector; Apr | Stratagene |
| pKS-ureB | ureB cloned into the KpnI-SacI site of pBluescript KS(+) | This study |
| pKS-ureB::KSF | KSF cassette cloned into the BamHI site of pKS-ureB | This study |
| pKS-ureBH136A | pKS-ureB with His136 replaced by Ala | This study |
| pKS-ureBH138A | pKS-ureB with His138 replaced by Ala | This study |
| pKS-ureBK219A | pKS-ureB with Lys219 replaced by Ala | This study |
a
Cm, chloramphenicol; Ap, ampicillin; Kan, kanamycin; Suc, sucrose; KSF, Kanr-sacB-PflaA (62).
b
American Type Culture Collection, Manassas, VA.