TABLE 3.
Primers used in this paper
| Name | Sequence (5′→3′)a | Restriction site |
|---|---|---|
| UreBF1 | GCTTCGGTACCCCTGAGAGAAGGCATGAG | KpnI |
| UreBR1 | GCATCGAGCTCCCGGCAACACTTGTCT | SacI |
| UreB:H136AF | GTGGTATTGACACAGCTATCCACTTCATTTCAC | |
| UreB:H136AR | GTGAAATGAAGTGGATAGCTGTGTCAATACCAC | |
| UreB:H138AF | ATTGACACACACATCGCCTTCATTTCACCCC | |
| UreB:H138AR | GGGGTGAAATGAAGGCCATGTGTGTGTCAAT | |
| UreB:K219AF | GGTGCGATTGGCTTTGCAATCCACGAAGACTGG | |
| UreB:K219AR | CCAGTCTTCGTGGATTGCAAAGCCAATCGCACC |
a
Primers were purchased from Integrated DNA Technology, Coralville, IA.