Figure 2.

Rli1 ribosome-dissociation activity has essential functions in diverse cellular processes, including ribosome maturation and release of stalled ribosomes. Schematic representation of a maturing ribosome formed by 60S (blue) and pre-40S (orange) subunits. Mature 40S subunits are depicted in pink. A faulty 40S subunit is represented by pink parallel lines. Class I release factors Dom34 (orange) and eRF1 (dark blue) dissociate ribosomes together with Rli1, whose NBDs are represented in green, and the FeS domain in brown. The “clamping” factor Stm1 is depicted in purple. The no-go decay mRNA contains a secondary structure represented by a red rhombus. The non-stop mRNA lacks an in-frame stop codon, depicted by a dashed line. Arrows indicate ribosome association-dissociation flow.