Figure 2.

Olfactory ensheathing cells (OECs) and GnRH neurons express Reelin receptors apolipoprotein E receptor 2 (ApoER2), very low-density lipoprotein receptor (VLDLR) and the adaptor protein Disabled 1 (Dab1) in explants. (A–F) Periphery of explants double-labeled for OECs (A–C) and GnRH cells (D–F) with indicated antibodies. (A) VLDLR (red) is expressed by OECs (green, S100, arrows). In addition, VLDLR highlighted positive cells associated with OECs (boxed area A1) that did not stain for OEC markers. OECs (B,C Sox10, red) co-expressed ApoER2 (B, green, example shown by arrow) and Dab1 (C, green, example shown by arrow). Sox10 negative Dab1 positive cells (arrowheads) were associated with OECs. GnRH cells (D–F, red, white arrows), co-expressed VLDLR (D, green) ApoER2 (E, green) and Dab1 (F, green). Numerous non-GnRH positive VLDLR cells (D2, green arrows) and Dab1 cells (F2, green arrow) were detected, consistent with OEC expression described above. TUJ1 was used to stain both GnRH cells and olfactory axons in (E,F). Although GnRH cells co-expressed ApoER2, olfactory fibers were devoid of staining (no fibers detected in E1 and fibers only red in E2). Dab1 was absent from the majority of olfactory fibers as well (F). Scale Bar = 100 μm in (A–C). Scale Bar = 50 μm in (D1,E1,F1); 100 μm in (D2,E2,F2), double labeled images of (D1,E1,F1), respectively. Insets in (D2,E2) correspond to black arrow in lower magnification.