Skip to main content
. 2018 Aug 6;9:1817. doi: 10.3389/fimmu.2018.01817

Figure 3.

Figure 3

Suppression of allogeneic T-cell activation and proliferation by culture-expanded invariant natural killer T (iNKT) cells. Function of culture-expanded iNKT cells was tested in a MLR. Dendritic cells (recipient) and allogeneic major-mismatched CD3+ T cells (donor) were used as stimulators and responders at a 1:1 ratio, respectively. Increasing numbers of expanded iNKT cells from donor origin were added to the culture. (A) Representative dot plots of CD3+ responder T cells and (B) pooled data from four independent experiments of CD25 expression on live CD3+ responder T cells and CD4+ and CD8+ subsets at day + 3. Dot plots are gated on live lymphocytes and iNKT cells were excluded by prior CFSE staining. (C) Representative CFSE dilution histograms and (D) pooled data from three independent experiments of live allogeneic CD3+ T cells and CD4+ and CD8+ subsets at day + 7. Error bars indicate SEM, *p < 0.05, **p < 0.01, ***p < 0.001.