FIGURE 2.

A Survey of tal gene diversity in a set of Malian Xoo strains. (A) Genomic patterns of tal content as determined by Southern blot. The genomic DNAs were digested with BamHI prior to electrophoresis on a 1% agarose gel. Following transfer, bands corresponding to tal sequences were detected with a probe encompassing part of the N-terminal coding region of TalF from MAI1. The names of tal genes corresponding to MAI1 fragments are indicated on the left. (B) Detection of talC in Malian Xoo genomes. A portion of the N-terminal region of tal coding sequences was amplified by PCR with primers flanking a segment that is specifically deleted in talC and separated on a 1.5% agarose gel. Detection of a shorter 152 bp product as compared to the predominant ∼224 bp product is indicative of the presence of talC. Purified plasmid DNA containing talF or talC and genomic DNA from strains KACC10331 (Korea) and PXO86 (Philippines) or BAI3 (Burkina Faso) were used, respectively, as negative or positive controls. The single gel image was broken down into three subpanels for assembling the figure. For each of them, the first lane was loaded with a 100 bp Marker and the numbers on the left indicate fragment size in bp. Strain names are indicated on top of the blot and gel images.