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. 2018 Jul 1;34(6):477–485. doi: 10.1089/jop.2017.0126

FIG. 2.

FIG. 2.

(A, B): Dual PIK3 and MEK inhibition with PD0325901+ZSTK474 cocktail effectively prevents AKT and ERK phosphorylation. Western blot analysis of 661W cells (A) and ARPE-19 cells (B) treated for 24 h with PD0325901+ZSTK474 and analyzed with antibodies against MEK and PI3K signaling proteins. (C, D) MEK inhibitor PD0325901 prevents baseline ERK phosphorylation and PI3K inhibitor ZSTK474 prevents baseline AKT phosphorylation in retinal cells. Western blot analysis of 661W cells (C) and ARPE-19 cells (D) treated for 24 h with PD0325901 or ZSTK474 (E, F) and analyzed with antibodies against PI3K and MEK signaling proteins. GADPH is shown as the loading control.