Figure 2. . Hybri-Care culture medium promotes the formation of more defined acini-like structure than Dulbecco's modified Eagle medium/F12 culture medium.

Optical microscopy images of MCF-12A cell embedded in Matrigel after 14 days of culture. When using Dulbecco's modified Eagle medium/F12 medium to maintain cultures, resulting multicellular structures seem smaller, less defined and looser (A–C, arrowheads) than cells maintained in Hybri-Care medium (D–F). In Hybri-Care medium, acini are bigger, rounder, more defined and more compact. Scalebars: 250 μm.